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Search
  • HOME
  • ABOUT US
  • REAGENTS
    • i-DNA Brand
    • Other Brands
  • SERVICES
    • Antibody Purification Service
    • Bacterial E.coli Competent Cell Preparation
    • Custom Peptide Synthesis
    • Custom-Made Monoclonal Antibody
    • Custom-Made Polyclonal Antibody
      • Rabbit Polyclonal Antisera Against PEPTIDE
      • Rabbit Polyclonal Antisera Against PROTEIN
    • DNA Paternity Testing
    • DNA Purification
    • ELISA
    • Gene Synthesis
    • Laboratory Animal Diagnostic
      • Comprehensive Health Monitoring For Rat & Mouse
      • Infectious Disease PCR Testing For Rat & Mouse
      • Mouse and Rat Serology Testing by MFIA
      • Custom Animal Serum, Whole Blood and Plasma
    • Preparation of Custom Buffers and Solutions
    • Protein Profiling
      • Protein Profiling Service for Cytokines
      • Disease-Specific Protein Profiling Service
      • Protein Profiling Service For Transcriptional Factors
    • GST- or His Taq Protein Purification Service
    • RNA Gene Expression
      • RNA Gene Expression For Multiplex
      • RNA Gene Expression For Single Plex
    • Site-Directed Mutagenesis
    • SNP and Genotyping
    • Subcloning
  • INSTRUMENTS
  • LAB ANIMALS
  • NEWS & EVENTS
  • CONTACT US

GST- or His Taq Protein Purification Service

i-DNA Biotechnology (M) Sdn Bhd > GST- or His Taq Protein Purification Service

GST- or His Taq Protein Purification Service

Catalog: i-CS5

Provide by Customer:
  • Plasmid DNA containing the target gene fragment.
  • Information of material provided and instruction for the serivce.
Procedure:
  • Amplification of target gene fragment from the plasmid DNA provided.
  • Cloning of amplified PCR product into a shuttle vector for DNA sequencing and restriction digest check.
  • Subcloning of the correct PCR product into an appropriate E.coli expression vector for GST-or His-fusion protein, depending on customer’s preference.
  • Screening for the correct and high fusion protein expression bacterial clones.
  • Large scale (1-5L) bacterial culture and protein purification of His-tagged protein using Ni-NTA column or GST-tagged protein using glutathione column.
Deliverables:
  • 10µg recombinant protein expression plasmid DNA and correspondin bacterial stock.
  • 5mg of fusion protein with >80% purity based on SDS-PAGE gel analysis.
  • Documentation of the project.
  • Turnaround time is 4-5 weeks.
Bacteria E.codiv Competent Cell Preparation
Custom Peptide Synthesis
Custom-Made Monoclonal Antibody
Rabbit Polyclonal Antisera Against PEPTIDE
Rabbit Polyclonal Antisera Against PROTEIN
DNA Paternity Testing
DNA Purification
ELISA
Gene Synthesis
Comprehensive Health Monitoring For Rat & Mouse
Infectious Disease PCR Testing For Rat & Mouse
Mouse and Rat Serology Testing by MFIA
Custom Animal Serum, Whole Blood and Plasma
Preparation of Custom Buffers and Solutions
Protein Profiling Service for Cytokines
Disease-Specific Protein Profiling Service
Protein Profiling Service For Transcriptional Factors
GST-or His Taq Protein Purification Service
RNA Gene Expression For Multiplex
RNA Gene Expression For Single Plex
Site-Directed Mutagenesis
SNP and Genotyping
Subcloning

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i-DNA Biotechnology (M) Sdn Bhd
A-1-6, Pusat Perdagangan Kuchai,
No. 2, Jalan 1/127,
Off Jalan Kuchai Lama,
58200 Kuala Lumpur.


t : 03 7982 0322
f : 03 7987 4566

e : info@i-dna.com.my

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